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anti saa1 2 antibody  (R&D Systems)


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    Structured Review

    R&D Systems anti saa1 2 antibody
    Anti Saa1 2 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+saa1+a2/Mouse+Serum+Amyloid+A1%2FA2+Antibody/pm41916996-438-46-48
    Average 93 stars, based on 58 article reviews
    anti saa1 2 antibody - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Serum Amyloid A1/Toll-Like Receptor-4 Axis, an Important Link between Inflammation and Outcome of TBI Patients
    Article Snippet: Thirty micrograms of protein were resolved by SDS–PAGE and transferred to Immobilon-P membranes (Millipore Corp., Billerica, MA, USA). .. Membranes were incubated with the primary antibodies anti-iNOS (1:1000; Cat No. sc-650, Santa Cruz Biotechnology, Dallas, TX, USA), anti-SAA1/A2 (1:1000; Cat No. AF2948, R&D Systems, USA) or anti-β-actin (1:50,000; Cat No. A3854, Sigma-Aldrich, Madrid, Spain). .. Appropriate peroxidase-conjugated secondary antibodies (1:5000; Cat Nos. sc-2354 and sc-2357, Santa Cruz Biotechnology, USA) were used to detect proteins by enhanced chemiluminescence.

    Article Title: Serum Amyloid A1/Toll-Like Receptor-4 Axis, an Important Link between Inflammation and Outcome of TBI Patients
    Article Snippet: Brains were extracted, embedded in OCT media, frozen at −80 °C, and sectioned in 15 μm thick slices using a cryostat (CM 1100, Leica Microsystems, Madrid, Spain). .. Brain sections were fixed with 4% paraformaldehyde dissolved in PBS for 10 min and washed three times with PBS every 5 min. After 2 h of blocking with 20% goat serum, tissue was incubated overnight at 4 °C with the primary antibodies anti-APP (1:1000; Cat No. 36-6900, ThermoFisher Scientific, Waltham, WA, USA) or anti-SAA1/A2 (1:100; Cat No. AF2948, R&D Systems, USA) diluted in PBS with 0.3% Triton X-100. ..

    Blocking Assay:

    Article Title: Serum Amyloid A1/Toll-Like Receptor-4 Axis, an Important Link between Inflammation and Outcome of TBI Patients
    Article Snippet: Brains were extracted, embedded in OCT media, frozen at −80 °C, and sectioned in 15 μm thick slices using a cryostat (CM 1100, Leica Microsystems, Madrid, Spain). .. Brain sections were fixed with 4% paraformaldehyde dissolved in PBS for 10 min and washed three times with PBS every 5 min. After 2 h of blocking with 20% goat serum, tissue was incubated overnight at 4 °C with the primary antibodies anti-APP (1:1000; Cat No. 36-6900, ThermoFisher Scientific, Waltham, WA, USA) or anti-SAA1/A2 (1:100; Cat No. AF2948, R&D Systems, USA) diluted in PBS with 0.3% Triton X-100. ..



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